Service Details

• Fluorometry Analysis refers to the measurement and study of fluorescence emitted by a sample when it absorbs light or electromagnetic radiation. This technique is widely used in biology, chemistry, and environmental sciences to identify and quantify substances, often with high sensitivity.

1. Fluorescence: A phenomenon where a substance absorbs light (usually UV or visible) at one wavelength and emits light at a longer wavelength.

2. Excitation and Emission:

Excitation wavelength: The wavelength of light used to excite the sample.

Emission wavelength: The wavelength of light emitted by the sample.

3. Fluorophores: Molecules that fluoresce upon excitation. Examples include:

Organic dyes (e.g., fluorescein, rhodamine)

Proteins (e.g., GFP – Green Fluorescent Protein)

Quantum dots and other nanoparticles.

Steps in Fluorometry Analysis

1. Sample Preparation: Ensure the sample is optically clear and free of contaminants that could interfere with fluorescence.

2. Excitation: Illuminate the sample with a specific wavelength of light to excite the fluorophores.

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